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Given that the rate of new microtubule end association with cell tips is ∼1/min [14], and ∼10 cables per cell tip [4], we estimate the lifetime of cortical foci to be several minutes, consistent with reported FRAP experiments of Tea1p [15].
Notice that at the 0.05 level, we would have expected 13.4 foci to be significant under this null hypothesis.
The HE-stained slide was also used as a reference for selection of tumour foci to be analysed in the FISH assay.
This staining revealed REC8- and CREST-positive foci to be associated and juxta-posed from day 5 to day 9 11, although somewhat irregularly.
The TURP specimens examined in our previous study allowed three tumour foci to be sampled in the vast majority of cases, thus allowing Ki-67 assessment in multiple regions of tumour.
In addition, a lack of any observable tendency for foci to be "paired" in neighboring cells (i.e., no "dueling") implies at the single-cell level that there is no "Tit-for-Tat" strategy operating in S. marcescens.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com