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In line with this hypothesis RNA foci mark distinct sites of genes or regulatory sequences that become repressed during XCI.
If Vilya foci mark CO, one does not expect a high colocalization frequency between gH2AV and Vilya (may be only 30% or less).
The great majority of blocked ori1 foci colocalized with DnaQ (81%), confirming that the replisome foci mark the location of the blocked replication forks.
DOI: http://dx.doi.org/10.7554/eLife.02445.014 RPA2 foci mark the presence of single-strand DNA (ssDNA), a consequence of end resection.
Assuming the times of appearance and disappearance of foci mark replication initiation and termination, respectively, then this value for S phase, determined for timelapse cells growing on slides, is slightly longer than our previous estimate of ∼55 min for cells growing in the same liquid medium (Wang et al., 2005).
Although the answer was not unequivocal, it does support the idea that residual DNA repair foci mark cells that are likely to die.
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(A ) Yme2-GFP coaligns with ERMES foci, marked by Mdm34-TdTomato, at mitochondrial division events.
Indeed, the majority of Lam6-GFP signal co-localized with ERMES foci (marked by Cherry-Mdm34).
Histochemistry indicated that the metastatic foci (marked by arrow head) are derived from colorectal cancer (Fig. 4d).
The magic wand tool based on the 8-flood fill algorithm was provided to indicate PCa foci marked on the background image.
Consistent with the previously published work, we observed that over time a subset of Yme2-GFP labeled nucleoids persistentlYme2-GFP labeledenucleoidsES foci, marked by Mdm34-tdTomato.
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