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The CD diet promoted the number of GGT+ foci above levels in control livers.
Compounds decreasing percentage of high-intensity foci above 25% of the control were selected as "hit" candidates, for further validations.
Correcting this rate for ∼71% efficiency of detection of DSBs as foci (above), provides a rate of 0.021 ± 0.008 DSBs per cell division.
Just as for Neurl4, GFP Neurl4 was concentrated apically, although more clearly enriched at two foci above each nucleus (Fig. 4B,C).
In a typical batch, there were ∼100 200 images and the user could rapidly check the automated algorithms have counted the foci accurately, graph the numbers of red or green foci, filter colocalizing foci above a certain threshold and export the filtered results to a tab-delimited text file for further analysis.
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