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There also was no difference demonstrated between the 4 focal sections of the ligaments that were examined.
All the confocal pictures represent single focal sections.
The images presented are single focal sections.
15 focal sections with 0.3-µm spacing were acquired.
An Olympus 100×/1.4 NA objective, 1 × 1 binning was used to create stacks of 15 focal sections and 0.2-µm spacing.
Images of slices through several micrometer thick sections along the z-axis, combined with 3-D rendering of composite focal sections, revealed Ago2 within the nuclear compartment.
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The pinhole device was adjusted to capture fluorescence of one airy unit in one focal section.
However, in any given NMJ section, there may be two or more clusters of spots that are disjoint due to the intervening regions being out of focus at that focal section.
Figure 1 Typical series of focal optical sections along the vertical axis (control MSCs in this very case).
Quantitative analysis of fluorescence intensities was carried out after performing the background removal in each image using the image processing Sigma Scan Pro 5.0 (SPSS, Chicago, IL, USA) software.Assessment of actin fiber distribution within the thickness of a cell was performed using z-stacking (serial focal optical sections along the vertical axis) (Figure 1).
In contrast, post-seismic slip areas (Iinuma et al., 2008) are distributed in the shallow low-velocity zone in the northeastern part of the focal area (cross sections a d).
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