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Exact(2)
The pinhole device was adjusted to capture fluorescence of one airy unit in one focal section.
However, in any given NMJ section, there may be two or more clusters of spots that are disjoint due to the intervening regions being out of focus at that focal section.
Similar(58)
There also was no difference demonstrated between the 4 focal sections of the ligaments that were examined.
All the confocal pictures represent single focal sections.
The images presented are single focal sections.
15 focal sections with 0.3-µm spacing were acquired.
Images of slices through several micrometer thick sections along the z-axis, combined with 3-D rendering of composite focal sections, revealed Ago2 within the nuclear compartment.
An Olympus 100×/1.4 NA objective, 1 × 1 binning was used to create stacks of 15 focal sections and 0.2-µm spacing.
A ×60/1.25 water-immersion objective (Olympus) was used to collect 3-D stacks of 17 focal sections (an entire nucleus of 6 8 μm) with 0.5 μm focal step, once a second for 200 seconds.
To determine whether the plasmids localizing to the periphery co-localized with the nuclear pores, Nup82-3sfGFP intracesy traces (N > 50 cells) were measured along the nuclear envelope in equatorial focal sections of the nuclei containing a single labeled acentric DNA circle at the rim.
Figure 1 Typical series of focal optical sections along the vertical axis (control MSCs in this very case).
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