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We approximate the effect of the aberrations by consolidating them in a single surface we call the focal phase plate (FPP).
Immunostaining of renal biopsy revealed that NET components of citrullinated histone, MPO and PAD4 were concurrently deposited both around fibrinoid necrosis in necrotizing glomerulonephritis in the early focal phase of ANCA-associated polyangiitis and along an interlobular arterial wall.
On the basis of the immunostaining results, this work provided convincing evidence for the concurrent appearance of NET components of citrullinated histone, MPO and PAD4 in necrotizing lesions of the early focal phase of ANCA-associated polyangiitis.
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Remarkably similar elongated tubules were also seen by the focal fluid-phase endocytosis assay, validating the endocytic nature of these compartments.
To further monitor the subsequent transport and fate of nascent endocytic compartments, we performed the focal fluid-phase endocytic assay while extending the duration of time-lapse confocal imaging.
For SLIM, the back focal plane of the phase contrast objective is projected onto a liquid crystal phase modulator which is used to impart additional phase shifts to the un-scattered light in increments of π/2.
For SLIM, the back focal plane of the phase contrast objective is projected onto a liquid crystal phase modulator (LCPM).
The lens L2 (f2 = 90 mm) is arranged in a way that its rear focal plane and the phase plate coincide, producing a reversed image of the phase object on the CCD element of the camera.
Focal audiences for Phase One of implementation (the first year) were the general population aged 15-49 years, with a specific focus on singles and cohabiting couples aged 15-29.
During this habituation phase, focal and all-animal sampling (Altmann, 1974; Lehner, 1996) were used to identify the composition of each group.
Indeed, our focus at present is on using the attributes of HIFU to treat in a focal manner with phase II prospective ethically approved trials close to completion (Ahmed et al, 2007;, 2009).
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