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Briefly, fluorescence imaging was carried out using a cooled fast 14-bit EMCCD camera (iXonEM+ DU-860, Andor Technology) equipped with 50 mm focal length objective.
For example, with a nominally 18-mm focal length objective lens, we measure an axial scan range of 1450 µm with a resolution of 75 µm.
With a nominally 3-mm focal length objective lens, we measure an axial scan range of 60 µm with a resolution of 3.8 µm.
Reflectance images were acquired with a Cascade CCD camera (type 512B; Photometrics, Tucson, AZ, USA) equipped with a lens pair of a 135-mm and a 50-mm focal length objective (NA 0.46, 2.7× magnification) using custom software written in Labview (National Instruments, Austin, TX, USA).
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We present, as well, measurements of the spherical aberration coefficient Cs=(108.7±9.6) mm and minimum achievable focal step δf= 87.6±1.4) nm for the specially designed long-focal-length objective lens of this microscope.
Bubbles were observed through the chamber window at ×40 magnification by means of a Wild™ M10 stereomicroscope with a long focal-length objective.
The spectrometer consists of a 75 mm focal length collimating objective, a 1200 l/mm diffraction grating, and a 150 mm objective which focuses the light onto the CMOS detector (spL4096-140km, Basler).
In this paper, to solve the above-mentioned problem and obtain long focal length focusing objective with short total measuring system length, the wide wavelength band telephoto superachromatic objective with less than 3/4 telephoto ratio is introduced.
The lateral extension of the dCV (ddCV) (estimated from the typical size of the speckle in the objective pupil (dspeckle) by ddCV ≈1.22 λfobj/dspeckle [ 27, 28], where fobj is the focal length of the objective) increases monotonically and seems to saturate at large depths (Fig. 5(c), FOV 1000 µm and Fig. 5(d), FOV 530 µm).
The magnification, sometimes referred to as magnifying power, is determined by dividing the focal length of the objective by the focal length of the eyepiece.
LFDisplay needs information on the microlens array pitch, focal length, the current objective magnification, NA and the specimen index of refraction.
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