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Imaging with WSPR allowed observation of the focal contacts without the need for immunostaining.
The WSPR experiments also indicated that TGF-βs influenced the distribution of focal contacts.
In conclusion, we showed that focal contacts are mechanoeffectors and that hyper-mechanical stimulation could up regulate focal contacts size as compared to hypo-mechanical that down regulate clusterization.
Cyclic deformation induced a biphasic response comprising new focal contacts formation followed by their clustering in ROS cells.
The RGD-conjugated peptides promoted human dermal fibroblasts spreading with well-organized actin stress fibers and focal contacts.
Cells of spindle and polygonal morphologies were randomly distributed on the random fibers, with no focal contacts observed.
On the other hand, p-FAK (Tyr-407), an inhibitory form of FAK, accumulated in the focal contacts of Ti-treated cells.
Pyk2 phosphorylated on Tyr-402 colocalized with paxillin in focal contacts of untreated cells, while it was barely detected upon exposure to particles.
In contrast, when seeded on laminin-5-functionalised native films, epithelial cells formed only very diffuse focal contacts, but adhered via hemidesmosome formation.
Cytoskeleton disassembly was associated with a reduction in overall cell adhesion area, characterized by fewer centrally localized focal adhesions and shorter focal contacts at the periphery.
Well-organized actin stress fibers and vinculin accumulated focal contacts were observed in the cells attached on the AG73 EF1zz (molar ratio = 1 9 chitosan membrane.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com