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Taken together, these observations identify four levels of organization in the perimysium: (i) Perimysial Junctional Plates that constitute the focal attachment between the perimysium and the myofibers, (ii) collagen plexi attaching adjacent myofibers, (iii) a loose lattice of large interwoven fibers, and (iv) honeycomb tubes connecting two tendons.
The podoplanin expression was most pronounced in areas of focal attachment and in small membrane protrusions (microspikes).
Yellow arrows in Fig. 4b reveal the firm focal attachment between the deflated cell and the neighbouring PLA nanofibres.
Our results showed that, in our patients, focal attachment of the membrane was associated with better final vision.
OCT imaged a focal attachment of the hyaloid to the center of the fovea with intact inner foveolar cyst and a tiny defect involving the outer retinal layer.
In his study of 174 eyes, focal attachment was seen in 26% of eyes and 67% eyes had a globally attached ERM.
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These data are consistent with non-motile cells, unaffected by contact guidance, which are spreading uniformly and increasing cytoskeletal tension on their focal attachments, leading to the formation of SMAs.
To further quantify cell-matrix attachment, focal adhesion formation was induced by Forskolin after serum starvation.
The observation that small focal adhesions appear directly after the disappearance of the membrane blebs suggests a role in focal adhesion formation and attachment.
Laminin-332 interacts with two major epithelial integrin α3 β1 and α6 β4, and promotes the formation of two separate types of attachment structure, focal adhesion and stable anchoring contacts (Carter et al, 1990).
The net effect of the combined action of PKC and Src is to increase the levels of Rac activity relative to RhoA, which inhibits stress fiber formation and reduces focal adhesion and cell attachment favoring a more migratory phenotype required for αvβ3-dependent invasion.
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