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Increasing the concentration of the FN solution results in a dense network-like structure of FN on the methyl terminated surface and large molecular aggregates that cover the whole surface for the more hydrophilic surfaces (Figure 2).
Different fibronectin (Fn) solution concentrations were tested in interaction with the quasi-crystalline/amorphous Ti45Zr38Ni17 composite.
Surface saturation is clearly reached for Fn solution concentrations of 100 150 μg mL−1 for both surfaces.
Fibronectin (FN) solution 1 mg/ml was purchased from Sigma.
After 20 min incubation at room temperature, the FN solution was aspirated from the channel.
After the activation of Sulfo-SANPAH, 200 μL of 50 μg mL−1 FN solution in PBS was spread on the activated PAA substrate.
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Plates were washed with PBS-BSA and the Fn solutions were added and incubated for 2 h with shaking.
Briefly, FN in solution was adsorbed onto a PDMS stamp used for microcontact printing with a surface pattern consisting of 50 μm × 20 μm rectangles (Fig. 1a).
Hep binding to FN in solution converts the FN dimer from a globular to an elongated structure and thus can enhance growth factor binding by presentation of cryptic binding sites31.
Plasma FN in solution alone will not polymerize [ 120] and will not form a three-dimensional matrix in the absence of cells [ 116].
When transferred from a physiological buffer to a supersaturated solution, Fn's crossed-over dimeric arms separate, indicating a weakening of the electrostatic interactions which otherwise stabilize the compact conformation of the protein.
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