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We used two fly media with different nutritional conditions.
Kite flying and media management to lower people's expectations were routinely deployed among the black arts of politics.
During lifespan observations over 60 days, we changed fly media and assessed survival every 2 days.
For the three-camera tracking assays, vials containing standard fly media stained with blue food coloring (Kroger brand) were used, as the blue colored background was required for efficient tracking.
We are grateful to Dr. Guillaume Chanfreau for detailed and insightful comments on the manuscript, Dr. Christos Delidakis for the generous donation of the Groucho monoclonal antibody, Dr. Richard Jones for the wing disc ChIP protocol and to Dr. Larry Marsh for the histone deacetylase inhibitor fly media preparation protocol.
Fly crosses were carried out at 25°C and grown on standard fly media.
All fly crosses were carried out at 25°C and grown on standard fly media.
Flies were reared on standard cornmeal fly media at 22°C or 25°C.
All Drosophila stocks and crosses were maintained on standard fly media.
Yeast is the main source for micronutrients and amino acids in standard fly media.
We also found that larvae fed 20E supplemented fly media become smaller in size.
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