Simon's fruit-fly protocol, for instance, demands that various minutiae be precisely tuned — lighting, temperature, humidity, and even whether you've cut new vials from their plastic bags far enough in advance to let out the stale air.
In addition, we developed the multi-layer Meta-Protocol framework, which allows for on-the-fly protocol discovery and negotiation, distribution of protocol specifications and components, and automatic protocol implementation in any programming language.
Genomic DNA was isolated from adults of D. yakuba and D. santomea with a single fly squish protocol [21].
Genomic DNA was isolated using the single fly preparation protocol described previously (Yeh et al. 2006).
DNA extraction from individual flies, PCR protocols, and allele scoring followed methods outlined in Gockel et al. [ 36].
Hemolymph was isolated from offspring of pUAST WT (WTb) and control w flies crossed with Act5C-gal4 (10 flies) and gmr-gal4 (30 flies) using the protocol described previously (36).
Briefly, whole flies were extracted with the single-fly squish prep protocol [21].
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Genomic DNA was prepared from 141 isochromosomal lines of Drosophila pseudoobscura using a single-fly DNA extraction protocol (Gloor and Engels 1992).
We found that 2 hr after treatment of 0 7 day old w 1118 flies with the standard injury protocol, both antibiotic-fed flies and flies without antibiotics had higher levels of expression of the AMP genes Attacin C (AttC), DiptB (Diptericin B), and Metchnikowin (Mtk) than equivalently treated flies that were not subjected to the standard injury protocol.
It's a matter of protocol to fly their home country's flag during their visit," said Smith.
