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Captured images were sent to the computer and the fly number in each tube was counted by a custom-made ImageJ macro.
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The funny thing is that stable fly numbers this summer seem low so far.
It's a slow process of birth control, reducing tsetse fly numbers generation by generation.
*Expect an increase in fly numbers in barns as flies seek warmer habitats to escape cooler outside temperatures.
Aggressive implementation of these measures led to a 166-fold and 546-fold reduction in sand fly numbers and complete eradication of the local rodent reservoir, Rhombomys opimus, inside the camp during 2006 and 2007, respectively.
It has recently been introduced in several areas in California and has brought down fly numbers by as much as 96%, says Kevin Hackett, national program leader for biological control at ARS headquarters in Beltsville, Maryland.
Fly numbers in experimental and control groups were similar in the two weeks post-hatching.
Fly numbers and Trypanosomosis incidence were significantly lowered in the treated area compared with the control.
ATP was measured using an Orion microplate luminometer (Berthold Detection Systems GmbH) and data normalized to fly numbers.
Individual flies can lay hundreds of eggs, which can result in a large increase in fly numbers in a short period.
Fly numbers fluctuate through the summer and decline in October, but the decline is less dramatic and defined than the spring increase.
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