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We retained months where fly mortality is typically highest: January and October, immediately prior to rainy season onset.
To provide enhanced sensitivity and discriminating power to the fly mortality tests, CI was adapted to be performed in Drosophila (see Materials and methods for details).
Temperature extremes, particularly above ∼36°C and below ∼10°C, also lead to adult fly mortality through starvation and water loss via respiration [9], [10].
Low humidity — moisture levels directly related to precipitation (Fig. 1 (D) — is also involved in fly mortality, though the exact mechanism is not clear [9], [11], [12].
Our trials conducted with the bscN mutant of strain K56-2 shows a partial difference in fly mortality pattern when compared to the parental strain (Figure 5C).
Nevertheless, the presence of wasps is correlated with higher fly mortality (lower larva-to-pupa survivorship) during the larva-to-pupa stage [wasp effect P = 0.0065; GzLMM including wasp treatment, infection state, and fly strain (random effect); Fig. 1B].
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They die like flies, (infant mortality rates, 40 per 1,000, compared to a national average 26.6 per 1,000) and those that survive, survive because they possess excessive vitality and a capacity of adaptation to the degradation with which they are surrounded".
Interestingly, Wang et al. reported that the TG-knockdown flies increase mortality after entomopathogenic nematode infections, suggesting TG-dependent clot formation works as an important effector by helping to prevent septic infections [16].
Many people want to become young again, and when for some flies a mortality plateau was seen at very old age, claims were made that also for humans the mortality has a maximum somewhat below 100 years.
The fly's high mortality and fitness impacts are of conservation concern for vulnerable and declining species of Darwin's finches.
The expression of the hsp70-GFP and hsp22-GFP reporters could also be assayed longitudinally and correlated with fly behavior and mortality.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com