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Author Contributions J.L.P. completed all experiments and analyses, with assistance on lifespan, PCR genotyping and general fly maintenance from J.J.McG., and on the development and application of behaviour data analysis software for measures of sleep intensity (the Drosophila Activity Monitor Data Crunching Macro) from K.P.K. J.L.P. and R.A. wrote the manuscript.
Fly maintenance and infection assays were conducted as described previously [8], [36].
We thank Mariana Mata and Sarah Pedigo for help with fly maintenance and general laboratory assistance at Iowa.
Vials were then kept under the same conditions as described above for general fly maintenance.
We also thank Ville Hoikkala and Mikko Merisalo for their help in fly maintenance.
We thank Aysha Hill-Williams and Alex Blasetti for fly maintenance, Yoganand Sundaravadanam for bioinformatics support, and Matthew Johnson and Peter Wilson for sequencing support.
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