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We also document stronger induction of herbivore-induced plant volatiles (HIPV) in S. altissima plants given previous exposure to the fly emission relative to unexposed controls.
However, our previous study did not exclude the possibility that the fly emission (or its residue) might also deter insect feeding via direct effects on the herbivores.
Building upon this work, the current study elucidates additional effects of exposure to the fly emission on S. altissima defense responses, as well as the direct effects of the emission itself on insect feeding.
However, the existence of such enhancement does not exclude the possibility that the fly emission might also have directly deterrent effects on insect feeding that contribute to the subsequent reduction in herbivory.
Together with our previous findings, these results provide compelling evidence that reduced herbivory on S. altissima plants exposed to the emission of male E. solidaginis reflects an evolved plant response to olfactory cues associated with its specialist herbivore and does not involve direct effects of the fly emission on herbivore feeding behavior.
Reproductive output of S. altissima plants suffers significantly from galling by E. solidaginis[ 41]; thus, detecting reliable cues associated with impending attack, such as the male fly emission, could provide plants with an advantage in their defense against E. solidaginis attack [ 15].
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The resulting classifiers are tested with data collected by the same spectrometer mounted on an aircraft flying over the emission stack.
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