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As shown in Figure 1F, CrPV loads increased exponentially in the days that precede fly death.
A dose response curve further showed that the rate at which hemocytes were depleted was directly proportional to the dose of CrPV injected (Figure 3C D) and that complete hemocyte depletion immediately preceded fly death (Figure 3D).
An excess of virgin flies was heat shocked to account for fly death during experimental treatment, which was on the order of 15%%.
This strain is pathogenic to Drosophila, meaning that it is able to grow rapidly and cause fly death when introduced into the hemocoel.
Alternative approaches, such as the fungal burden in immunocompetent D. melanogaster, are much more difficult to quantify and, in this case, would not be as good a virulence indicator as fly death.
To calculate the fly virulence index (FVI), data on fly death rates were fitted to Weibull distributions as suggested for C. albicans (Glittenberg et al., 2011), and the time at which 50% of the animals had succumbed to the infection was calculated (LT50).
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Fly deaths were recorded every other day.
From week 3, fly deaths were greater in controls than 670 nm exposed flies and they remained so at each time point until week 11 12, when all flies were dead in both groups.
The age specific mortality rate (μ x) was calculated using WinModest by binning the days over which deaths were counted (since fly deaths were recorded every other day) such that μ x = (-ln(N x + δ x / N x )) / δ x or P x = N x + δ x / N x and μ x = -1/δ x ln(P x )), where N xis the number of flies alive at day x and δ x is the bin size [ 2].
"The EC225 is a flying death trap," one oil worker with 20 years' experience told the BBC.
Still, aviation authorities in former Soviet nations insist their civilian airliners are as safe as Western counterparts, despite the feeling that the planes, with their cramped and often dingy interiors with bad upholstery, are little more than flying death traps.
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