An intact cerebellum is essential for on-the-fly corrections of posture and gait (Armstrong 1986; Morton and Bastian 2006; Morton et al. 2004).
Therefore, we chose the third and fourth eluted standard peptides IGDYAGIK and TASEFDSAIAQDK as the reference peptides for on-the-fly corrections, and the detection time window was set at 16 min so that no reference could be missed even with large retention time shift.
A TSQ Vantage triple-quadrupole mass spectrometer (Thermo Fisher Scientific, San Jose, CA) was utilized for the scheduled LC-MRM analysis with on-the-fly correction for retention time (RT) shift.
Volumetric scanning or on-the-fly correction of eye movements with an eye tracker could reduce the artifacts of transverse movements.
Thus, without the on-the-fly correction of retention time shift, the transitions of these two peptides would not be captured in the predicted retention time window.
First, on-the-fly correction generates new solid k-mers, which serve as starting nodes in the next pass; second, because of repeats in the sequence, the search for a path can proceed to different parts of the graph depending on which end of the region it is started from.
To correct for systematic retention time shift during the scheduled LC-MRM analysis, we incorporated an on-the-fly correction in our MRM detection workflow, during which the instrument automatically detects the shift in retention time for predefined reference peptides and adjusts, in real-time, the retention time window for subsequently eluted targeted peptides.
Reference ions were used for on the fly mass correction.
PET/MRI can be used for on-the-fly motion correction.
The resulting enriched kinase peptides were analyzed by two scheduled MRM runs with a 6 min retention time window and on-the-fly retention time correction strategy.
During a Ravens game, if Rice is on the field or if he and Montgomery are too far apart on the sideline, they will use their own set of hand signals to make comments or corrections on the fly, Rice said.
