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Affymetrix BPMAP files were remapped using xMAN (Li et al., 2008) and the latest version of the fly assembly (UCSC dm3/Flybase release 5.8).
Nevertheless, our results also clearly indicate that the Hessian fly assembly was largely valid and that most errors are associated with the contigs containing the greatest number of Qs.
In addition, the percentage of Qs in the Hessian fly assembly (0.32) was lower than that observed in maize (11.0) [ 15], B. rapa (15.0), catfish (7.3), and Nile tilapia (9.6).
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The fly (genome assembly version dm3) and worm (genome assembly version WS220) ChIP-seq and RNA-seq data were generated by modENCODE consortia.
We were unable to annotate s-adenosylmethionine synthetase from the Hessian fly genome assembly.
The Hessian fly genome assembly contained 6 orthologues of Snmp1 and one Snmp2.
We annotated 32 Obp genes in the Hessian fly genome assembly, including two pseudogenes.
The current Hessian fly genome assembly contains genes for 122 ORs, 28 GRs, 39 IRs, 7 SNMPs, and 32 OBPs.
We performed Illumina sequencing and subsequently mapped the sequenced reads to the current Hessian fly genome assembly to predict transcripts and analyze gene expression profiles.
Illumina mRNA-Seq reads were aligned to house fly genome assembly v2.0.2 and annotation release 100 (Scott et al. 2014) using TopHat2 v2.0.8b (Kim et al. 2013) and Bowtie v2.1.0.0 (Langmead et al. 2009) with the default parameters.
Although the general arrangement of the region differed significantly to that of D. melanogaster, a search of this sequence in the recently published B. cucurbitae (melon fly) genome assembly (NCBI accession GCA_000806345.1) revealed the same arrangement of miRNAs in B. dorsalis, except for the region where the two duplicated miRNAs were found.
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