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Spiroplasma titers were quantified at 14 days of fly age.
In all experiments, fly age ranged from 10 to 16 days.
However, additional optimization of the protocol may be necessary before the technique can reliably estimate fly age.
(D ) Fluorescent microscopy images depicting SYTO-9 stained Spiroplasma in Drosophila hemolymph at 7 days (D1 ) and 21 days (D2 ) of fly age.
This approach controls for possible effects of environmental variables that change as a function of variable weather patterns or fly age.
Developmental time may be important in nature; in Drosophila, a rapid development reduces fly age at the first breeding and so would be at a premium.
Similar(53)
This fluorescence tends to disappear as the fly ages (only residual fluorescence is evident after 3 weeks).
It would appear instead that they start at a low titer and are expanding as the fly ages but never reaching their "limit" imposed by the DAG levels.
The fact that Dca showed no expression changes between the reproducing and young females suggests that the detected transcriptional changes may not be connected to fly aging.
Reviewer 2: Avanesov et al describe a metabolome analysis of fruit fly aging, using both a standard and lifespan extending restricted diet.
Together, these fluorescence and TEM data suggest that progressive changes in the epidermis occur as the fly ages, and may represent one of the earliest reporters of insect tissue aging.
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