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These differences in autophagic flux were confirmed by analyzing puncta formation (Fig. 5C and D).
Changes in the autophagy flux were confirmed by an increase in the immunofluorescence of the LC3b punctae upon CQ treatment (Fig. 3D, S5B).
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And by comparing with the traditional centralized regularization (CR), the necessity of respectively regularization for each inversing heat flux is confirmed by using different regularization parameters.
The presence of autophagic flux was confirmed using bafilomycin A1, which led to a striking accumulation of the unprocessed LC3-II.
13 Autophagic flux is confirmed if LC3-II is further increased in the presence of these inhibitors, but often, an additional molecule called p62 (also called SQSTM1) is evaluated to confirm this conclusion.
Tzb-promoted dynamics of autophagosomal formation (i.e. punctate pattern of LC3) and increased autophagic flux (i.e. p62 degradation) were confirmed with Western blot analyses (Figure 8).
These immunofluorescence results were confirmed by determining the autophagic flux by immunoblot.
The significant flux effect is confirmed for the weld metal (nickel concentration ⩾1.35%) which is mainly due to development of reversible temper brittleness.
The observed astrophysical neutrino flux has been confirmed with >6σ significance with both events starting within the detector (all flavor) and events traversing through the Earth (νμ charged-current).
Considering TOC and nitrate fluxes, it is confirmed that the worst situations were encountered for very high flow rates, namely for TOC exportation during surface runoff which was related to heavy rains or floods.
The activation of ion fluxes by HAMLET was confirmed in DLD1 cells preloaded with Ca2+ or K+ fluorophores (figure 4G).
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