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To investigate whether IFN1@ is a direct activator of autophagic flux, we detected microtubule-associated protein 1 light chain 3 α (LC3) conversion (LC3-I to LC3-II) in the absence and presence of lysosomal protease inhibitors.
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This result suggested that the theoretical L-arabinose metabolic flux was higher than we detected in BSW3AP.
To test this hypothesis, we detected the autophagic flux in our cell model.
Interestingly, DRAM1 is partially contributing to this specific ER α-driven autophagic pathway, as we detected a reduced autophagic flux in ER α-expressing cells after DRAM1 knockdown (Supplementary Figure 5a).
Using a cocktail of lysosomal inhibitors, we were able to corroborate that this sensor can measure autophagy flux as detected by the accumulation of LC3-II by western blot analysis, or the massive accumulation of autophagosomes (yellow dots) that did not reach lysosomes.
No increase in X-ray flux was detected by GOES at the time of the event.
It is associated with a magnetic flux rope detected with the NEAR spacecraft magnetometer.
No differences in flux were detected across dietary treatments for phenol, indole, skatole, or 4-methylphenol (P > 0.23).
Moreover, aeration is further reduced when permeate flux lower than the average design flux is detected, if the facility is operated under variable fluxes.
A lowering of the groundwater table, increased upper soil moisture, reduced 2 m air temperature, and enhanced latent heat flux were detected by the end of the simulated period, and the changes at the land surface were related linearly to the water demands.
It should be mentioned that the method used is analogous to 2-deoxy-2-[F]fluoroglucose (FDG) positron emission tomography (PET), with the exception that the actual metabolic flux is detected as opposed to simple probe uptake.
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