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To monitor carbon flux, we analyzed the expression of genes encoding key enzymes in gluconeogenesis, glycolysis and TCA cycles (Figure 3).
Hence, to clarify if the increased level of LC3-II was due to an increase of autophagy induction and/or to a block of the autophagic flux, we analyzed p62 protein, which is a read-out of a bona fide autophagic process.
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Using E-Flux-MFC, we analyzed the gene expression data from this experiment.
In order to assess the rate of autophagosome formation (i.e., autophagic flux), we also analyzed LC3-II levels in the presence of the lysosomal protease inhibitors E64d and pepstatin A, which block LC3-II/autophagosome degradation.
In search of first wall concepts that can withstand surface heat fluxes beyond 2 Mwem2, we analyzed advanced "monoblock" designs using coolants and materials that offer more attractive long-term performance.
However, the turnover rate with 200 B approximates the turnover rate of the flux configuration with 100 B. We analyzed the fluxes from the time point 0 to 5, where the network is not in steady state yet, for the 100 B case.
To determine whether pharmacological inhibition of PHGDH displayed similar effects on autophagy in ECSLCs, we analyzed autophagy flux following treatment with low (4 µM) and high (10 µM) doses of CBR-5884.
To address this issue further we analyzed autophagic flux by 'enhanced-mitophagy'.
Therefore, we analyzed autophagic flux in LNCaP cells using the lysosome inhibitor chloroquine (CQ).
To investigate the relative roles of Alr and Ddl in the mechanism of DCS action in more detail, we analyzed metabolic flux through the d-Alanine pathway following DCS inhibition by employing H-isotopically labeled alanine.
Briefly and according to Klionsky et al., we analyzed autophagic flux using GFP-LC3 fluorescence by adding lysosomal protease inhibitor (CQ) to cells expressing GFP-LC3, and monitoring changes in the number of dots.
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CEO of Professional Science Editing for Scientists @ prosciediting.com