Exact(1)
Beyond the expected shift from FA (four-fold down) to carbohydrate (CHO: 2-fold up) (p < 0.001) at both preloads, these hearts depicted a 44% lower glycolytic flux rate when expressed relative to that of pyruvate decarboxylation at the higher preload (p <0.05), suggesting a mismatch between cytosolic and mitochondrial CHO metabolism.
Similar(7)
Previously measured flux rates, when available, are a particularly valuable guide for further predictions [ 15, 20, 21].
On the other hand, the open system of perfusion allows the constant addition of defined substrate concentrations and calculation of metabolic flux rates, when metabolic steady-state conditions are achieved.
This allowed establishing the flux rate boundaries of each reaction when the network is used to produce one particular metabolite.
The reduced cost signifies the amount by which the objective function (e.g. growth rate) would increase when the flux rate through a chosen reaction was increased by a single unit [ 77].
In this case, in silico IC50 values are calculated by estimating the maximal flux rate carried by the target reaction when growth rate is set to 50% of its maximal value.
A much slower drop-off in signal is clear versus the reflectance in depth, and when the ratio of these numbers is determined, the effective flux rate is plotted.
Analysis of the root H+ flux rate using a noninvasive technique revealed a differential pattern of H+ fluxes along the lateral rice roots when treated with HA (Figure 3A).
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