Your English writing platform
Discover LudwigSuggestions(1)
Exact(6)
For both biological systems, each EFMset represents a functional network with specific behaviour in which the coefficients of flux efficiency of the reactions are calculated.
The relative difference of measured fluxes (ΔF) was compared with that of the flux efficiency of the corresponding reaction in the enlarged network.
For instance, in C. glutamicum, the flux efficiency of the phosphofructokinase (PFK) decreased on sucrose compared to glucose while the experimental flux did not change.
The overall changes in experimental fluxes observed with respect to the nitrogen source are confirmed by the change in the flux efficiency of the corresponding reaction in the enlarged network.
The coefficient of flux efficiency of each reaction of the five EFMsets (Sg, Sf and Ss for C. glutamicum and Nm and Naa for the B. napus) was calculated.
As defined by [ 17], the flux efficiency of each mode is related to the investment required to produce the enzyme needed to establish the mode, approximated by the sum of all absolute fluxes, because for comparable metabolite concentrations, the flux through a reaction scales linearly with the enzyme concentration.
Similar(54)
Among the 16 fluxes measured experimentally (9 internal, 3 uptake fluxes and 4 fluxes towards output metabolites), the direction of the change (sign of ΔF ) of 15 fluxes are accurately predicted by the change in flux efficiencies of the EFM analysis, and only one, the malic enzyme flux (Vme) is not.
For B. napus, the main changes in experimental fluxes observed using the two nitrogen sources (mineral and amino acids) are confirmed by the change in the flux efficiencies of the corresponding reaction in both the core network and the enlarged network describing the metabolism of a heterotrophic plant cell.
This structural analysis shows specific behaviour in agreement with experimental conclusions; future work will attempt to generalize the potential interest of EFMs and the intrinsic value of the coefficient of flux efficiency to correlate with metabolic fluxes or "omics" data.
The coefficient of flux efficiency is calculated from the matrix of EFMs of N elementary modes (rows) and P reactions (columns).
While most of the differences of flux efficiency are well correlated with the experimental fluxes, some of them do not fit the experimental results.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com