Exact(4)
Firstly, evidence of particle internalization was obtained by transmission electron microscopy and conventional flux cytometry techniques.
A direction that has not been explored for eliminating at least the problem of heterogeneous cell shapes, is the generation of protoplasts and their analysis by flux cytometry.
Lymphocytes count for all participants used flux cytometry methods implemented with BD FASCOUNT automate (BD Biosciences, Le pont de Claix, France).
A possible solution to the problem of cell shape heterogeneity might be the generation of protoplasts and their analysis by flux cytometry, but from our experience the necessary digestion of the cell wall would trigger stress responses critically biasing the observations.
Similar(4)
Some techniques are difficult to adapt to the emergency context (multiplex tests, ELISA or high-flux cytometry).
We provide here a detailed protocol for our recently published method using mCherry-EGFP-LC3 to quantify autophagic flux by flow cytometry and to sort subpopulations of cells based on their relative levels of autophagic flux.
We have therefore developed a method to measure autophagic flux by flow cytometry and have used it to successfully sort cells based on their relative levels of autophagic flux.
When measuring flux by flow cytometry it is highly advisable to have software either during the acquisition of flow data or in the subsequent analysis that is capable of handling a ratiometric calculation as a derived parameter.
Related(1)
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