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Each individual run was corrected for sample absorption, photon flux, buffer scattering, and detector homogeneity.
In this assay, various nucleotides at a concentration of 1 mM were added individually to GsuK-containing liposomes loaded with high [K+] followed by mixing with the flux buffer containing radioactive Rb.
Each tested nucleotide was added to a 30 μl aliquot of the liposomes collected after the buffer exchange step followed by the addition of 56 μl Rb flux buffer (450 mM Sorbitol, 10 mM HEPES, pH 7.4, 50 μM KCl, and 5 μM RbCl).
For influx studies, Caco2BBE cell monolayers were washed once in 150 mM choline Cl, 10 mM HEPES pH 7.4 and then unidirectional apical membrane sodium uptakes were determined in flux buffer (130 mM Choline Cl, 5 mM KCl, 1 mM MgCl2, 2 mM CaCl2, 15 mM HEPES pH 7.4, 20 mM NaCl with 1 μCi/ml [NaCl]) for ten minutes.
Similar(56)
Second, it is possible that increased urea cycle flux may buffer the appearance of ammonia in the blood by detoxifying ammonia that would otherwise accumulate as a by-product of amino acid catabolism.
It is interesting to note that the large Ca2+ flux to the buffers, primarily to the stationary buffer, fluctuates significantly less than the outflux of Ca2+ ions from the cleft.
The As flux during GaAs buffer layer growth corresponds to a flux gauge reading of 1 ×10−5 Torr.
Because of the possible importance of the ligand conditions in assessing the physiological functioning of the Ca pump, we have looked at (Ca + Mg -dependent ATP hydrolysis in resealed ghosts with buffered ATP and Ca levels and at the active Ca flux with a buffered ATP level.
This paper presents a simplified method and data filtering algorithm for measuring electrophysiological, transmembrane H+ flux activities in buffered media based on the self-referencing microsensor technique.
It is likely that excretion serves as a buffer for flux capacity to these intermediates, allowing the cell to adjust formate consumption capacity according to need, rather than maintaining a high constitutive capacity.
The long-range interactions buffer the flux through other methyltransferases when one methyl-transferase is downregulated.
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