Sentence examples for flux blockage from inspiring English sources

Electron microscopy data corroborates this hypothesis, as it shows that the VP-16-induced autophagic vacuoles in the RB-silenced group (siRNA-RB plus VP-16) are frequently filled with electron dense material, similar to the cytosol (indicated by white arrows), possibly representing vacuoles containing non-degraded material due to autophagic flux blockage.

For example, 109 genes out of 1322 have a minimum FBA score of 0, corresponding to a complete blockage of fluxes; however, only 48 of them (44%) are truly essential.

It is shown that flux decline is due to blockage of the membrane surface by lateral growth of the deposit rather than the hydraulic resistance of a cake building up at the membrane surface.

This protein is degraded in autophagolysosomes and, thus, its accumulation indicates a blockage in autophagic flux.

With this mechanism, HSPB8 facilitates ARpolyQ autophagic clearance and relieves the blockage of autophagic flux (37).

Increased LC3-II and blockage of autophagic flux in lymphoblasts from patients with WDR45 mutation has been demonstrated implicating defective autophagy in the disease pathogenesis [ 4].

Similar to the effect of BECN1 and ATG7 knockdown, the blockage of autophagic flux by protease inhibitors E64d and pepstatin A attenuated the increase of cell viability induced by EEF2K silencing (Fig. 4C).

This mechanism has been studied further by Ma et al. [68], who showed that AuNPs that are taken up and accumulate in lysosomes induce autophagosome accumulation through the blockage of the autophagy flux.

In fact, while cytoplasmic soluble ARpolyQ (testosterone-untreated) impairs the ubiquitin proteasome system (UPS), testosterone-induced ARpolyQ aggregation correlates with normal UPS activity, induction of autophagy (6– 8, 32– 36) and also with a blockage of the autophagic flux (37, 38).

Any one metabolic network can also effectively compensate for the blockage of individual reactions by rerouting metabolic flux through other pathways.

Taken together, our data suggest that RB silencing causes a blockage on the VP-16-induced autophagic flux, which is followed by apoptosis in GBM cell lines and in cancer stem cells.