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A path flux analysis showed that O generated by the plasma was the critical species for extinction enhancement.
Global gas holdup profiles in conjunction with the drift flux analysis showed that pressure does not have a significant impact at elevated liquid flow rates as bubbles tend to follow the convective current of the liquid.
Metabolic flux analysis showed that the flux through the pentose phosphate (PP) pathway, one of the main pathways that produce NADPH, was reduced significantly in the recombinant strain when compared to that of the parent strain.
A reaction flux analysis showed that 99% of C6H5SiCl3 was generated from the insertion reaction, 74% of SiCl3H was decomposed into SiCl2 and 91% of SiCl2 was inserted into C6H5Cl to form C6H5SiCl3.
Flux analysis showed that the acetaldehyde dehydrogenase reaction (acetaldehyde + CoA + NAD+ → acetyl-CoA + NADH + H+), involved in toluene metabolism, was used to generate NADH within the cell.
Metabolic flux analysis showed that NADPH requirements in the cytosol remain nearly the same in these experiments due to constant biomass growth.
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Reaction flux analysis shows that dominant consumption channels for T135MB oxidation are H-abstraction reactions to form 3,5-dimethylbenzyl radicals, while reactions with O/OH radicals to generate 1,3,5-trimethylphenoxyl/1,3,5-trimethylphenyl and ipso-addition to form m-xylene play minor roles.
In the growth conditions studied, metabolic flux analysis shows that the total cytosolic NADPH demand is nearly constant at a rate of 21 mmol/Cmol/h, irrespective of the ethanol fraction or the cysteine synthesis route (see Fig. 1).
Flux variability analysis showed no variability in the fluxes of the pathways discussed below - both in the uninfected and the infected models.
Flux variability analysis showed the differences between maximum and minimum fluxes for acetate, formate and ethanol were 0.0003, 0.011 and 0.011 mmol per 1 mmol of glucose.
Flux variability analysis showed that the acetate yield was not different between the maximum and minimum fluxes.
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