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Malic enzyme activity was also detected in cell extracts, consistent with the flux analysis results.
Flux distributions in S. oneidensis predicted by the L-HCM compare very favorably with 13C-metabolic flux analysis results reported in the literature.
Furthermore, the activity and transcript level of the lipogenic enzyme glucose-6-phosphate dehydrogenase, the rate-limiting enzyme of the pentose phosphate pathway, were also about 2-fold higher than that of the wild-type; these data corroborate the flux analysis results.
This result was basically in accordance with the metabolic flux analysis results mentioned above.
However, according to our C flux analysis results, the oxPPP flux remained largely constant up to 48% ethanol.
This prototype platform can be a springboard for advanced and broad-scope modeling of complex biological systems by integrating other "omics" data or C- metabolic flux analysis results.
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All of the metabolic flux analysis result supports the feature of high butanol/acetone ratio when adopting cassava substrate.
The instationary C flux analysis resulted in fluxes comparable to previously obtained from NMR datasets of proteinogenic amino acids, but allowed several additional insights.
CytoSEED also provides the capability of switching among the various flux variability analysis results available for a given model from the Model SEED web-server.
By protons release and Ca2+ flux analysis NA resulted in a rapid response, which was inhibited by WS433 and CPZ.
Based on the preliminary analysis results, metabolic flux and gene transcriptional analysis were conducted to verify the assumption and to elucidate the mechanism about high butanol/acetone ratio in cassava-based fermentation.
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