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The obtained data were applied for the respirometric 13C metabolic flux analysis in Part II.
Results from isotope based metabolic flux analysis in combination with considerations on NADPH metabolism clearly exclude the existence of Pgi isoenzymes in C. glutamicum strain DSM5715.
Then the dynamic element flux analysis in the on-the-fly reduction is integrated to determine the active non-QSS species at each time step of the computation.
In this minireview the potential of MFA to increase the chances of success in metabolic engineering of plants is presented, recent progress related to engineering and flux analysis in central metabolism of plants is discussed, and some recent advances in flux analysis methodology are highlighted.
As one of the critical linkers and the similar glutamate specific consumption rate of FB-L and FB-H, we investigated the glutamate metabolic flux analysis in FB-L and FB-H.
This interesting notion should be further addressed by metabolic flux analysis in future studies.
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RNA sequencing makes it possible to carry out some "High-flux" analysis in I. indigotica.
This can have an important impact on the use of spline smoothing for flux estimation in metabolic flux analysis (as in [ 13], for example).
Monte Carlo methods have previously been applied to metabolic flux analysis (MFA) in order to handle inaccuracies in data and model [ 11].
The reduced network suggested by our flux analysis is in agreement with the reduced network given in [ 10].
The 13C-metabolic flux analysis performed in our previous study showed that active SAM biosynthesis in the Kyokai 6 strain should be derived from enhanced ATP regeneration with high TCA cycle flux (Hayakawa et al. 2015).
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