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Following bead loading, lysis buffer was introduced into the array, and the array was then immediately sealed by introducing fluorinated oil.
Continuous streams of separated immiscible liquid phase compartments (water and fluorinated oil, Figure S1 in the Supporting Information) are generated in a microfluidic device which was realized as a polydimethylsiloxane (PDMS) chip, or a fused silica capillary T-junction.
Fluorosurfactants, or fluorinated surfactants, soluble in both water and fluorinated oil, are also less volatile and more stable at high temperature than hydrocarbon-based surfactants due to the stability of the carbon fluorine bond.
To evaluate the performance of the modified PFPEs, we compared PFPE1 Tris1.0 with the unmodified PFPE in generating emulsions in a flow-focusing-based droplet generator, using 2 wt % of surfactant dissolved in Novec HFE-7500 fluoilnased oil as the continuous phase.
Due to the symmetric arrangement of the fluorine atoms, each fluorinated oil or amphile molecule gives one sharp singlet 19F NMR signal.
Subsequent infusion of low surface energy lubricants (fluorinated oil Fluorinert FC-70 and ionic liquid 1-Butyl-3-methylimidazolium bis trifluoromethylsulfonyl)imide (BMIm)) into the porous coatings results in liquid-repellent slippery surfaces.
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The first heavy tamponade used was fluorinated silicone oil or fluorosilicone (FSiO), but its high rate of complications such as early emulsification and development of intraocular inflammation and PVR limited its use [ 6].
Our nanoparticle technology for stabilizing water-in-fluorinated oil emulsions has been licensed!
Fluorinated silicone oil (trifluoropropylmethylsiloxane or fluorosilicone-FsiO), which has a density of 1.30 g/cm, was the first heavy tamponade used.
They have been created by the combination of silicone oil and fluorinated alkanes in a homogenous solution.
In the hybrid air-electrodes, four kinds of CFx materials including fluorinated graphite, fluorinated carbon fiber, fluorinated coke and fluorinated black carbon were utilized as lithium insertion materials.
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