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Derivatives 1 and 3 have two pyrene moieties as a fluorescent unit.
Derivative 2 has only one pyrene moiety as a fluorescent unit.
One such tether incorporates an embedded fluorescent unit providing a C-mannosyl multivalent epitope offering potential applications through further conjugation or immobilization.
A series of novel cellulose derivatives bearing pyrene-based π-conjugated pendants as a fluorescent unit was synthesized from microcrystalline cellulose via carbamoylation followed by Sonogashira–Hagihara cross-coupling, and their photoluminescence and circularly polarized luminescence (CPL) properties were investigated in solution.
A 150 relative fluorescent unit (rfu) cut off was set to distinguish true fragment peaks from background fluorescence and other artefacts.
Addition of 0.2 mM cumene hydroperoxide induced an increase in lipid peroxide from 34682±341 RFU (relative fluorescent unit) (mean ± S.D). to 37027±1171 RFU (mean ± S.D).
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Fluorescence was expressed as relative fluorescent units and area under the curve (AUC) for the plot of fluorescent units over time.
The phagocytic index (PI) defined as the percentage of cells with internalized fluorescence × the mean relative fluorescent units internalized per cell (RFU/cell) was calculated as previously described (Gauthier et al., 2010).
The fluorescence intensities were calculated as relative fluorescent units (RFU) (relative to isotype control) using the LAF software and represented as RFU per unit area.
The GFP expressions of PGK-clones range from 87.76 to 656.90 arbitrary fluorescent units (AFU) after adjusting for background fluorescence, indicating that the GFP sequence was functional and that integration sites in the genome indeed influence expression.
A linear fit was then applied to the fluorescence at 0, 1, 2.5, and 5 μM, allowing the conversion from fluorescent units to micromoles.
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