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As a proof of concept, we demonstrate that a two-color fluorescent system can simultaneously and selectively detect two targets (thrombin and adenosine triphosphate) in a single solution.
As one of the most promising materials for the construction of desirable solid-state fluorescent system, new solid-state fluorescent host guest system, which consists of the imidazo[4,5-a]naphthalene-type fluorescent hosts 2 and sterically hindered guest molecules were designed and prepared.
All blood cultures were processed using the Bactec 9240 fluorescent system (Becton Dickinson).
Immunofluorescent labelling images were acquired with a fluorescent system (Axia Imager A1 microscope, AxioCam MRc5 camera, Axiovision software; Zeiss).
Capillary separation of sequencing reactions was undertaken by the Australian Genome Research Facility AGRFF) in Brisbane (Australia) using the Applied Biosystems fluorescent system.
With this fluorescent system western blot detection of HSP70 is reported to be quantitative across 4.3 orders of magnitude, from 5 pg to 100 ng (http://www.licor.com/bio/applications/quantitative_western_blots/quantification.html).html
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LED backlights are both brighter and thinner than the fluorescent systems used by most laptops currently.
4 Most LCD televisions employ "cold cathode fluorescent" systems, in which a single white light passes through colored filters onto a screen.
Thus, traditional molecular beacon-based fluorescent systems can be prepared.
To enhance the sensitivity to low abundance transcripts, this platform employs 60-mers oligonucleotides and a chemiluminescence-based approach to detection, with reduced background noise relative to standard fluorescent systems.
HRP activities were revealed with Tyramide-Alexa 488 or Tyramide-Cy5 (1∶100, TSA-fluorescent system Perkin Elmer).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com