Intracellular esterases' and ROS convert the non-fluorescence stain 2', 7' dichlorodihydrofluorescein (DCFH) to the fluorescent stain dichlorofluorescein (DCF).
A DAPI narrow-band fluorescence cube (BP330-385 nm/DM400/BA420) waselecteded for analyses with the fluorescent stain.
DAPI is a fluorescent stain that allows examination of nuclei in a fluorescence microscope for morphological assessment of changes during apoptosis [ 23].
Cytochemical localization of O 2 ·− presented that, a stronger intensity and larger surface area of the DHE-derived fluorescence appeared in leaves only infested by A. craccivora; while a small fluorescent stain was observed in the additionally inoculated cyanobacteria leaves.
This innovative technique is based on fluorescence in which a stream of solution containing the cells, labeled with a fluorescent stain, is illuminated with an LED.
Fifteen of 5,000 juvenile fish collected over the ensuing weeks had the tetracycline marker, which shows up as a fluorescent stain under a microscope.
DNA was visualized by treatment with the DNA fluorescent stain DAPI.
NR, a selective fluorescent stain for intracellular lipid droplets, has been used to visualise the skin penetration study successfully [47, 48].
Flow cytometric estimation of nuclear DNA content involved the MoFlo XDP Cell Sorter (Beckman Coulter, Fullerton, CA, USA), with propidium iodide (PI) as the fluorescent stain.
Figure 9c and d shows finally the expression of nano-DNA complex as confirmed by DAPI fluorescent stain with the help of fluorescent microscopy.
Thus, we used a DNA special binding fluorescent stain gel to get the images of point contacts with excitation wavelength in the range 400 530 nm [39].
