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Fluorescent sections were studied in a confocal laser fluorescence microscope.
Fluorescent sections were evaluated at 1.25× magnification with a special fluorescence microscope equipped with different filters appropriate for the used dyes (Leica® DM 6000B, Leica Microsystems CMS GmbH).
All fluorescent sections were imaged using a confocal microscope.
Fluorescent sections were examined with a confocal laser-scanning microscope (Zeiss LSM 510 Meta; Jena, Germany) using laser beams of 488 and 633 nm for excitation.
Fluorescent sections were coverslipped in polyvinyl alcohol with diazabicyclooctane (DABCO) as an anti-fading agent.
Fluorescent sections were embedded in Mowiol and analyzed under a fluorescent microscope (Zeiss, Jena, Germany).
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The DHE fluorescent section from WT-STZ mice depicted remarkably bright red fluorescence that signified probably high levels of ROS as compared to the WT, SOD2, and SOD2-STZ mice.
Fluorescent immunostained sections were examined under a Leica DMRA2 fluorescence microscope with Leica HC PL Fluotar 10 and 20X/0.5 NA dry objective, captured using Photometrics CoolSNAP HQ, (Photometrics, Friedland, Denmark), and processed with Metamorph 4.6 5 (Molecular Devices Sunnyvale, CA).
Fluorescent histological sections were analysed with the aid of an ApoTome® microscope and AxioVision Software (both Carl Zeiss Microimaging GmbH, Jena, Germany).
Fluorescent immunostained sections were used to obtain images with a Fluoview confocal laser-scanning microscope (Olympus Optical).
Confocal imaging of fluorescent stained sections was conducted using a Zeiss LSM 510 Meta confocal system and Zeiss LSM 510 software (Version 4.0 SP2) with multi-track scanning.
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