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(H2) GES-1 cells labeled with fluorescent secondary antibody.
(D2) MGcells celabeledeled with fluorescent secondary antibody.
The aqueous reaction medium contains, in addition to the derivatization product, a highly fluorescent secondary product: dansyl hydroxide (Bartzatt 2003).
Fluorescent secondary antibodies were from Jackson ImmunoResearch Laboratories.
Fluorescent secondary antibodies were used at 1∶500 in PBT.
Slides were then incubated in fluorescent secondary antibody from Invitrogen.
Alexa Fluor 488, 546, 633 and 647 fluorescent secondary antibodies were from Molecular Probes, Invitrogen.
After several washes, the cells were stained with corresponding fluorescent secondary antibodies for 1 hr.
Fluorescent secondary antibody was then added and incubated for 20 minutes at 4°C.
20 µl fluorescent secondary antibodies (Molecular Probes) were added for 2 h at room temperature.
16A11-immunoreactivity was detected using an Alexa red fluorescent secondary antibody (Molecular Probes).
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