Exact(14)
Using this mixer we have measured sub-millisecond fluorescence quenching kinetics while consuming fluorescent sample at rates no greater than 6 nl/s.
To summarize, metal-dielectric coatings are a versatile biophotonics tool that enable straightforward control of the axial fluorescence enhancement distribution by adjusting the distance of the fluorescent sample to the nanocoating or vice versa.
Fluorescence lifetime imaging microscopy (FLIM) generates an image from a fluorescent sample where each pixel measures the average lifetime from the corresponding focus spot.
To confirm the fluid dynamic simulations and to three-dimensionally characterize the microflow system we conducted confocal laser scanning and fluorescent sample measurements.
The mixer injects a narrow cylindrical stream (radius a < 1 μm) of fluorescent sample into a larger flow of diluting buffer that moves through a capillary (100 μm i.d). at a speed ∼20 cm/s, under laminar flow conditions (Re ≈ 14).
In ordinary confocal microscopy, a lens focuses a pulse of laser light into a small spot in a fluorescent sample.
Similar(46)
A similar filter approach could be used for obtaining fluorescence images for highly fluorescent samples.
Before the determination of FRET fluorescent samples were diluted to equal fluorescence intensity in the YFP channel with homogenization buffer.
It should be noted that fluorescence intensity decreases at increasing of the observation angle for the fluorescent samples without a photonic superlattice, such as photonic glasses with disordered silica globules (Figure 10a) and reference slabs of soda lime glass (Figure 10b).
DOC and FDOM samples were collected in precombusted amber glass EPA vials with teflon septa, acidified and frozen (freeze-thaw tests comparing refrigerated and frozen fluorescent samples from Emerald Lake indicated an average shift of 0.6% in fluorescence intensities and peak ratios, well within the range of instrument variability at these low fluorescence levels).
Two photon microscopes can look deep into fluorescent samples, typically 5-20 times deeper than other types of fluorescent microscopes.
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