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Consider for instance lensfree imaging of two fluorescent points that are directly located on a single pixel.
Under this condition, it is theoretically and practically impossible to resolve these two fluorescent points that fall within a single dummy pixel.
Prior to laser irradiation, each cell possesses 2 4 fluorescent points that correspond to the centrioles within the centrosome (Figure 2A,E).
In the case when two of these highly fluorescent points are located horizontally close, the initial closing operation would fill the distance in-between on the high pixel value.
However, the same two sub-pixel fluorescent points can be resolved from each other using lensfree compressive imaging if several pixels could detect weighted averages of their fluorescent emission.
Some parameters must be adjusted for each data type, e.g. the angle of the stripes or the size of the structuring element that initially removes the bright fluorescent points, but this is a very easy task for a person who is familiar with the programming environment and its syntax.
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This property can be efficiently utilizable in applications where the 3D position of singular fluorescent point source is to be determined.
Under those conditions, researchers can be relatively certain that each fluorescent point corresponds to only a single active molecule, thus making it possible to assign their positions with nanometer precision.
The result is a blurred image of a fluorescent point.
Figure 3 panels A and B show measured emission axial intensity profile from a fluorescent point source fixed on the coverslip under epi-illumination.
Let us consider a fluorescent point-like volume located at r f and embedded in a 9mm thick homogeneous diffusive slab (see Fig. 1).
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