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We discuss the estimation of particle diffusion statistics from closed-loop position measurements, and we present a generalized theory of fluorescence correlation spectroscopy for the case that the motion of a single fluorescent particle is actively tracked by a time-dependent laser intensity.
The flow has been experimentally studied by means of planar laser-induced fluorescence (PLIF) to visualize its structure, and particle image velocimetry (PIV) with fluorescent particle tracers to measure its velocity.
Localization of a single fluorescent particle with sub-diffraction-limit accuracy is a key merit in localization microscopy.
With this design, fabricated in SU-8, single fluorescent particle measurement was successfully carried out.
Sometimes, more intense staining is observed when the probe is attached to a fluorescent particle.
The motion of a single neutrally buoyant fluorescent particle is logged by the system, and the vectors of the trajectories are reconstructed into 3D coordinates.
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The images of green fluorescence (green fluorescent protein, gfp) and red fluorescence (red fluorescent particles, rfp) were taken after exposure times of 100 and 500 ms, respectively.
The images of green fluorescence (green fluorescent protein, gfp) and red fluorescence (red fluorescent particles, rfp) were taken after exposure time of 600 ms.
The fibre core is doped with scintillating fluorescent particles, which fluoresce when irradiated by ionising radiation, and cladded with PMMA.
Otherwise, 4X air objective is used to image the fluorescently labeled cells and fluorescent particles embedded in the collagen matrix.
We found that treatment of virions with CLR01 did not affect virion fluorescence or the number of fluorescent particles.
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