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FACE allows the derivation of correctly edited polyclones carrying a positive selection fluorescent module and the exclusion of non-edited, random integrations and on-target allele NHEJ-containing cells.
Results were documented using the fluorescent module of a Leica DM4000 microscope with Retiga camera (Leica, Wetzlar, Germany).
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Several affibodies directly labeled with fluorescent modules have been reported.
(F ) Schematic of Ire1 GFP and Ire1 mCherry with the fluorescent modules placed in the juxtamembrane region of the cytosolic linker.
To avoid interference with mutations in the linker, we relocated the fluorescent modules to the αF αEF loop in the Ire1 kinase domain.
We report here a short RNA motif that induces an efficient communication between the structural changes associated with the ligand-binding event of RNA aptamer and an optical response of a fluorescent RNP module.
To evaluate the neutrality of these loci, insertion/deletion mutants were produced, and to assess their functionality, a synthetic green fluorescent reporter module was introduced.
Accordingly, despite the insertion of a fluorescent protein module into the αF αEF loop, Ire1 still faithfully formed foci and recruited SpRU1A mRNA under ER-stress conditions and was indistinguishable from untagged wild-type Ire1 in splicing efficiency and growth under ER-stress conditions.
Grashoff et al. now report the development of a genetically encoded, fluorescent tension-sensing module capable of measuring mechanical forces across specific proteins in vivo.
We have shown the feasibility of this system in simultaneously visualizing tissue architectural morphologies (from the OCT module) and fluorescent molecule distribution (from the fluorescence imaging module) in ex vivo rabbit esophagus samples, and the results suggest future potential of the technology for in vivo and possibly clinical applications.
The design has a potential to eliminate the brightness enhancement film (BEF) for a red-green-blue (RGB) light-emitting diode (LED) or a cold cathode fluorescent lamp (CCFL) backlight module in the LCD systems.
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