Cells were incubated with 2 μM CMFDA at 37 °C for 30 min, following which fluorescent microscopic detection was performed under Leica DMLB Fluorescence Microscope.
Fluorescent microscopic images were visualized on an inverted microscope (Axio Imager M1 microscope, Zeiss, Göttingen, Germany).
The in vitro cellular uptake of SWCNT- KWKG 7- PEG 12/pDNA complex prepared with fluoreSWCNT- KWKG 7- PEG 12/pDNAaluated with fluorescomplexcroscopreparedrvation and flowithtometry.
The cells were then washed with PBS (3×5 min) and mounted with Vectashield with DAPI for fluorescent microscopic analysis under a Nikon E800 microscope.
Fluorescent microscopic images were obtained with a Nikon ellipse 80i microscope (Nikon Optical, Tokyo, Japan) and a Nikon Digital Sight DS-5 M camera using NIS-Elements F 2.30 software (Nikon).
The corresponding fluorescent microscopic images were obtained at 405, 488, and 635 nm using an inverted fluorescence microscope (Olympus, Southend-on-Sea, UK) [15].
Fluorescent microscopic observation revealed the successful modification of exosomes with CpG DNA by SAV-biotin interaction.
Fluorescent microscopic analysis revealed significant cell-to-cell heterogeneity in biopolymer accumulation.
Furthermore, anti-cancer drug testing was successfully demonstrated using the proposed culture platform and fluorescent microscopic observation.
Further the mechanism of cell death was analysed by fluorescent microscopic analysis and also by scanning electron microscopy.
Figure 4A shows the typical fluorescent microscopic observations in the five groups (wild-type, control, FUS alone, AR alone, and combined AR/FUS treatment; the detected plaques could be visualized with green fluorescence).
