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(h) The merged image of the bright field and the fluorescent image where fluorescence intensity was represented with a color spectrum.
Radioactive signals were quantified by phosphor imaging in a FLA-3000 fluorescent image analyser (Raytest, Fujifilm).
The fluorescent image was obtained with a fluorescence microscope (Olympus IX71, Japan) and confocal microscope (Carl Zeiss LSM710).
Radioactive signals on the imaging plate were quantified with a fluorescent image analyzer (FLA3000).
Thereafter fluorescent image of each gel was acquired using an Ettan Dige imager (GE Healthcare, Piscataway, NJ, USA).
Left, dark field image; and right, fluorescent image.
The fluorescent image shows the vascular network (RFP-HUVEC) with GFP-MDA-MB-231 cells.
(a) Fluorescent image of rigidity boundary between soft (1 kPa) and stiff (25 kPa) PAA matrices.
This fluorescent image shows endothelial cells embedded in a scaffold made of collagen.
The fluorescent image processing, base-calling and calculation of quality value were performed by the Illumina data processing pipeline 1.4.
(a) Fluorescent image of human red cells incubated in FSc1L-biotin (5 µM) and visualised with NeutravidinDyLight488 (100 µg/mL), (400× magnification).
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