Exact(25)
Samples were analyzed under a fluorescence microscope using a standard fluorescent filter set at 520 ± 20 nm.
Apoptosis was expressed as the number of apoptotic cells 10 ten fields with 1000 × magnification, using a fluorescence microscope with a standard fluorescent filter.
Depending upon the assay, one of the following three fluorescent filters was used: FITC HYQ fluorescence filter (460-500 nm); TX RED HYQ fluorescent filter (532-587 nm); and ultraviolet filter (325-375 nm).
The MagNest was then moved to Cell Tracks Analyzer II, which contains a semi-automated fluorescent microscope (4 fluorescent filter cubes) which captures images of fluorescently labeled cells that are immunomagnetically selected and aligned, covering the entire surface of the cartridge.
Stained cells were spotted onto a slide and cover slips were then mounted onto glass microscope slides and observed under fluorescence microscopy (Olympus BX51) using a 358 nm excitation and 460 nm emission fluorescent filter.
In an intraperitoneal tumor model, EGFP could be visualized endoscopically using a laparoscope with a fluorescent filter.
Similar(35)
Cells were viewed under a Nikon Eclipse 80i fluorescence microscope, using a ×20 objective and fluorescent filters appropriate for fluorescein and DAPI.
The intensity of relative fluorescence was monitored at every 0.004 min, for 80 min, on a Perkin Elmer LS 55 spectrometer with fluorescent filters (excitation 489 nm, emission 515 nm).
Therefore, we avoided using such interference based fluorescent filters, and instead, fabricated absorption based filters that have dyes coated on ultra-thin glass substrates (∼30 µm).
While the double fluorescent tracing method allows a direct qualitative assessment of the topographical distributions of RGC fibers from glaucomatous and control eyes, the use of different fluorescent filters precludes a precise quantitative comparison.
Slides were washed with 2xSSC/0.3% NP-40 at 73°C for two minutes, counterstained with DAPI and analyzed with an Olympus BX51 microscope, appropriate fluorescent filters and a CytoVision® imaging system (Genetix, San Jose).
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