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To verify that the punctate EGFP-LC3 fluorescence induced by the four chemicals represented autophagosome formation rather than, for instance, fluorescent drug precipitates, EGFP-LC3 fluorescence was examined at higher resolution by laser confocal microscopy.
If membrane fluorescence was seen, the media and fluorescent drug were removed from the well and the cells washed by the addition and removal of 400 µL HBS.
Another approach to detect resistant bacteria to antibiotics is using fluorescent drug analogs called reporter enzyme fluorescence [ 65, 66] or using drug fluorescent analogous with FRET pairs (fluorescence resonance energy transfer), that upon enzyme cleavage, the quenching molecule is eliminated and the fluorophore then emits light [ 67, 68].
Unlike the construction of fluorescently labelled proteins as simple ligand-binding biosensors [ 20– 26], the development of fluorescent drug sensors from TEM-type β-lactamases for in vitro drug screening is much more challenging because the catalytic activity of the modified β-lactamases must be largely conserved in order to mimic their wild-type form for drug testing purposes.
Anticancer fluorescent drug doxorubicin (DOX) was encapsulated into the liposomes.
The GO nano-sheets therefore have the potential to be used as fluorescent drug delivery carriers of specific size.
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Specificity is a particular problem in the determination of fluorescent drugs.
The movement of fluorescent drugs, NPs, or cells bearing NPs can be tracked and measured quantitatively with this protocol.
With its catalytically active nature and effective fluorescent drug-sensing function, the labelled TEM-1 V216C mutant can mimic its wild-type form as a molecular drug target for in vitro drug screening.
Cell sorting with a Becton Dickinson FACS II was used to determine the relative position of TGr cells and sensitivity to fluorescent drugs.
The photoluminescent behaviour of 6-coumarin has been recognized as a useful tool for the cellular uptake study of non-fluorescent drug molecules through fluorescence/confocal microscopy (Davda and Labhasetwar 2002).
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