For this study, we wanted a model probe that would have biological significance and good spectroscopic properties, and we chose calmodulin labeled with enhanced green fluorescent protein (eGFP-CaM).
injection of various photosensitizers and fluorescent dyes into the CAM for drug screening and prediction of clinical features.
In brief, the fragment containing enhanced cyan fluorescent protein (ECFP), calmodulins (CaMs), and M13 was fused to YPet and subcloned into pcDNA3.1 (Invitrogen, Carlsbad, CA) for mammalian cell expression by using BamHI and EcoRI sites.
The diffusion coefficients of green fluorescent protein (GFP), GFP-CaM, and GFP-CaMKII diffusing in 1× phosphate buffer saline were obtained by RICS and single-point TPFCS (see Materials and Methods for details on RICS and TPFCS).
G-CaMP2 is an FCIP composed of a circularly permuted green fluorescent protein (GFP) linked to calmodulin (CaM) and the CaM target sequence M13 [4].
Four different fluorescent dyes, calcein acetoxymethyl ester (CAM), carboxyfluorescein succinimidyl ester (CFSE), Vybrant DiO (DiO) and MitoTracker Green (MTG) were tested for labeling of NK target K-562 cells.
The blood vessels of the CAM were clearly fluorescent in all three lines of transgenic quail.
Furthermore, we found that photounbinding of CaM requires a fluorescent label but is not restricted to a specific label or wavelength [10].
Here, we report the spatial diffusivity and availability of CaM labeled with enhanced green fluorescent protein (eGFP -CaM, at basal and eGFP -CaMCat+, quantified basale novel fluorescent techniques of randelevated sCa2+ing spectroscopy and number and brightness analysis.
In highly magnified two-dimensional confocal fluorescent images (Fig. 2D), L1-CAM-ir profiles were observed in the cytoplasm that appeared as dots in the control DRGs.
