Sentence examples for fluorescent blot from inspiring English sources

In Fig. 4, endogenous CAML is shown on the fluorescent blot as a red band at 34 Kd.

Fluorescent blot was imaged on the Odyssey Infrared Imaging System (LI-COR Biosciences).

Blocking and primary antibody incubations for fluorescent blots were as previously detailed (Tavender et al, 2008).

To confirm that the RNA changes observed in YFP-16 mouse spinal cord resulted in corresponding changes at the protein level, expression levels for 2 proteins selected from the PCR array (caspase 1 and CCL3) were validated in YFP-16 mouse spinal cord using quantitative fluorescent western blot (Figure 1B,C).

Following optimisation, fluorescent dot blot analysis of transfection supernatants was shown to be a rapid method for analysing protein expression yielding similar results as western blot analysis.

(A ) Fluorescent Western blot analysis for histone H2B and H3 (green) using wild type embryos at 4 5 hr and sorted His C mutant embryos at 5.5 6.5 hr AEL, respectively.

(D ) Fluorescent Western Blot with α-Dlic antibodies revealing the levels of GFP-labelled Dlic compared to unlabelled, endogenous Dlic in the GFP Dlic extracts used for photobleaching analysis.

For fluorescent western blots, proteins were transferred to Odyssey nitrocellulose (Li-Cor Biosciences, Cambridge, UK), for traditional western blots, standard Protran membrane (Whatman, UK) was used.

For fluorescent western blots, equal amounts of samples were separated by electrophoresis on 8 12% SDS polyacrylamide gels and blotted onto a PVDF membrane (Millipore).

SMN protein levels, measured using sensitive quantitative fluorescent western blotting, declined rapidly over a period of several days following sample collection, especially when protein was extracted immediately and stored at −20 °C.

WldS protein levels in the brain were determined using quantitative fluorescent western blotting.