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Therefore, in studies of dynamic cellular processes, it would be ideal to be able to combine the capability of in vivo fluorescence video microscopy with the EM.
A key feature of this model that distinguishes it from other experimental models of intravital fluorescence video microscopy is that we record in 2-photon confocal mode.
The delivery of systemically injected polymer rhodamine conjugates to solid tumors was investigated by in vivo fluorescence video microscopy of ovarian tumors implanted in dorsal skin fold window chambers in nude mice, with and without local hyperthermia.
Elegant strategies have since been described for creating genetically encodable biosensors which may undergo a phosphorylation dependent change in FRET ratio and therefore can be used to directly visualize the temporal and spatial evolution of a given protein tyrosine kinase activity using fluorescence video microscopy [13], [14].
The fluorescence of the SF1+ neurons was detected by a fluorescence video microscopy camera (Olympus BX51).
The percentage of capillary vascularity (CV) was examined using intravital fluorescence video microscopy with a 10x objective lens [ 11].
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Implication of hemichannels permeability on PSB0474-induced ATP release was demonstrated by real-time fluorescence video-microscopy measuring the uptake of propidium iodide by intact urothelial cells in the absence and in the presence of MRS2578 or carbenoxolone.
C. sorokiniana mutants that exhibited a lower Chl fluorescence yield than WT when exposed to a saturating pulse of light were identified following UV mutagenesis using a fluorescence video-imaging system (Additional file 1: Figure S1).
In the cases where LPC projection was imaged, fluorescence videos were segmented by k-means (with 3 clusters) on the individual pixel traces of every run.
In the cases where cell somas were imaged (vPN1 imaging), fluorescence videos were segmented based on average intensity using k-means clustering, following which individual cell bodies were labeled as different region of interests.
NC-m4 cells were treated with the 4 neurotrophic factors for 2 wk as above, loaded with calcium indicator Fluo-4 and fluorescence videos made during perfusion with capsaicin or cinnamaldehyde (separate cultures).
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