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The high MFI values of fullerene 1 treated cells should be attributed to the strong fluorescent signal (relative fluorescence unit, RFU) emitted by fullerene 1-FITC, greater than that of fullerene 2-FITC, as determined using equimolar solutions of the FITC-labelled fullerenes in an in vitro cell-free system (RFU fullerene 1/RFU fullerene 2 = 4.1 ± 0.2).
eCFP, enhanced cyan fluorescent protein; eYFP, enhanced yellow fluorescent protein; FLIP, fluorescent indicator protein; FRET, fluorescence resonance energy transfer; GFP, green fluorescent protein; PBP, periplasmic binding proteins; PCR, polymerase chain reaction; RFU, relative fluorescence unit.
RFU relative fluorescence unit.
Relative fluorescence unit.
Results were expressed as relative fluorescence unit vs. time.
Data represented as relative fluorescence unit (RFU) / μg protein.
RFU represents relative fluorescence unit read in both of the culture conditions; undifferentiated versus differentiated.
The results are expressed as relative fluorescence unit per microgram of protein (RFU/μg protein).
Data reported as Ratio between relative fluorescence unit (RFU) of viable cells / RFU of apoptotic cells.
Relative fluorescence unit (RFU) was 14-fold higher in induced cells (5240 ± 1690) compared to non-induced cells (365 ± 233).
Caspase 3 activity expressed in relative fluorescence unit (RFU) was normalized against protein concentration of the cell lysates which was determined using Bradford Assay.
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