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Phantoms with lipid concentration 3% and 5% were also produced to further observe the fluorescence trend at high µ s′ values.
Of note, NO-dependent fluorescence of DAF-2/DA being due to irreversible oxidation of the probe molecule by NO, the fluorescence trend of DAF-2/DA is always positive, since it reflects accumulation of the fluorescent product of intracellular NO and the probe.
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Curve fit plots (shown as red lines) for inhibitor versus fluorescence plots were generated in Prism 6 for Mac OS X using the analysis parameters of "log inhibitor versus response variable slope (4 parameters)" to help illustrate fluorescence trends.
Interestingly, the fluorescence intensity trend follows the order: Fresh > ST > CD > MI.
With regard to the fluorescence, similar trends were observed when comparing the TMV samples; the underlying photophysics however differed.
Because at this stage we are interested in the oscillatory component of the myosin signal, the myosin fluorescence intensity minima trend was subtracted from the total myosin fluorescence intensity measurements.
An increasing trend in fluorescence intensity is visible in the images with inocula ranging from 10 to 10 CFU.
The changes in tryptophan fluorescence followed the same trend for the two LCDD proteins but LCDD-01 had higher fluorescence intensity throughout the whole assay when compared to LCDD-02.
As shown in Table 1 and Figure 4B, the results obtained for each vector such as % EGFP-positive cells and mean fluorescence intensity per cell trends were generally similar to the first experiment with donor 2. Also, vector copy numbers per cell were determined in this experiment and generally correlated with % EGFP-positive cells (Table 1).
The screen displays a graph reflecting the trend of the fluorescence intensity, giving users an intuitionistic view.
The trend based on fluorescence intensity of these samples follow the order: pure TiO2 > Mn-doped TiO2 (0.15%) > Mn-doped TiO2 (0.30%) > Mn-doped TiO2 (0.60%) > Mn-doped TiO2 (0.45%).
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